Lectin-binding patterns were observed with endothelial cells of human nasal mucosa according to the ABO blood group and they were observed to find the marker lectin differentiating fenestrated from continuous capillary. Mucosal specimens were
obtained
from the inferior turbinate in 85 patients who underwent the intranasal ethmoidectomy or septoplasty. All specimens were fixed in 10% buffered formalin and embedded in paraffin. Serial sections, 4㎛in thickness, were made and stained with blood
group
specific lectins(DBA, GSI-B4, UEA-I)and blood group non-specific lectins(SBA, PNA, RCA-I, WGA, Con-A) using avidin-biotin peroxidase method. Diaminobenzidine was used as a chromogen. The sections were counterstained with Mayer's hematoxylin.
Lectin-binding patterns were observed using Vanoxs light microscope.
UEA-I, WGA, RCA-I, and Con-A were labelled irrespective of blood group. UEA-I reaction was positive in capillary of deep portion, venule, and sinusoid. But no reaction was noted in capillary near gland.
These results suggest that lectin-binding patterns with endothelial cell of human nasal mucosa is non-specific to blood group and UEA-I can be as marker lectin differentiating fenestrated and continuous capillaries. Further study using
epon-embedding
method and immunoelectromicroscopy will be required. (Korean J Otolaryngo1 39 : 6, 1996)
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